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. 2009 Dec 30;84(6):2666–2674. doi: 10.1128/JVI.01562-09

FIG. 3.

FIG. 3.

HIV-1 accessory genes modulate MLV/GaLV Env restriction. (A) Schematic of HIV-1 packaging constructs ΔR8.2. (B) ΔR8.2 or its derivatives containing the accessory genes shown were cotransfected with reporter vector pSIN18.cPPT.hEF1a.EGFP.WPRE and either MLV Env or MLV/GaLV Env. Viral supernatant was transferred from transfected 293FT cells to 293T mCAT-1 cells, and infectivity was determined by flow cytometry. (C) Output of the same experiment with the infectivity of each construct expressed as the ratio of infections with MLV/GaLV Env to infections with MLV Env.