FIG. 5.
Vpu prevents MLV/GaLV Env from being incorporated into HIV-1 particles. (A) 293FT cells were transfected with HIV-CMV-GFP containing no Vpu (Vpu−), Vpu wt, VpuRD, or Vpu52/56, and MLV Env or MLV/GaLV Env. For the upper panel, Western blot analysis performed on the transfected cells and pelleted viral supernatants. The lower panel shows the infectivity output from the same experiment. Infectious units per ml were normalized to p24 levels. (B) The averages and standard deviations of infectious units per milliliter of six independent experiments with the same combinations are shown. Significantly different means for each Env treatment are indicated by unique letters (Tukey-Kramer HSD, P < 0.05).