TABLE 2.

CD8 T-cell epitopes for MHC-I tetramer synthesis

Pattern	ORF	Amino acids	Sequence	Function	Gene expressiona	MHC restriction	IC50 (nM)b	Tetramer
1	ORF9	283-291	SSWPPYNIL	DNA polymerase	E	Kb	120	No
1	ORF17	308-316	SAITNHAAF	Capsid protein	E	Db	81	No
1	ORF25	1208-1216	YSPENAAKL	Major capsid protein	L	Db	10	No
1	ORF39	209-216	LVLFYRPI	Glycoprotein M	L	Kb	149	No
1	ORF40	323-330	ITSMWPLL	Helicase-primase	L	Kb	102	No
1	ORF48	148-155	TNYKFSLV	Unknown function	E	Kb	10	Yes
1	ORF54	253-260	AVVQFIRV	dUTPase	E	Kb	45	No
1	ORF61	524-531	TSINFVKI	Ribonucleotide reductase, large subunit	E	Kb	32	Yes
1	ORF75c	176-184	SAIENYETF	Tegument protein/FGARAT	L	Db	10	No
2	ORF6	487-495	AGPHNDMEI	Single-stranded DNA binding protein	E	Db	14	Yes
2	ORF21	304-311	TGFRYSYM	Thymidine kinase	E	Kb	11	No
2	ORF39	167-174	FVYLFHFM	Glycoprotein M	L	Kb	16	No
2	ORF75c	940-947	KSLTYYKL	Tegument protein/FGARAT	L	Kb	11	Yes

^aE, early; L, late.

^bIC₅₀, 50% inhibitory concentration.