Effect of quercetin on the viability of prostate cancer cells. A: Normal prostate epithelial cells, (B) LNCaP, (C)DU-145, and (D) PC-3 prostate cancer cells were treated with increasing concentrations of quercetin for 24 hr and viability was determined by trypan blue vital dye exclusion. Viability was confirmed by a proprietary tetrazolium dye method using absorbance at 450 nm as described in the text: (E) normal prostate epithelial cells, (F) LNCaP, and (G) PC-3 cells. Values are mean ± SE of six experiments; *P < 0.05 and **P < 0.001 compared to treatment with dihydroquercetin which did not affect the viability of any of the cells in this study. [Color figure can be viewed in the online issue, which is available at http://www.interscience.wiley.com.]