Fig. 5.

Knockdown of Hsp90 gene expression by siRNA promotes apoptosis of prostate cancer cells. Cells were transfected with 40 nM Hsp90 siRNA, 40 nM scrambled control siRNA, or mock transfection using the transfection reagent without siRNA. Annexin V−positive cells were measured by flow cytometry after 24, 48, and 72 hr. A: Representative Western blots from three separate experiments which yielded similar results. Hsp90 siRNA induced marked suppression of Hsp90 expression in LNCaP and PC-3 cells, whereas β-actin expression was unaffected. Hsp90 siRNA enhances apoptosis of (B) LNCaP and PC-3 prostate cancer cells as measured by annexin V-positive cells. Results are mean ± SE of six experiments; *P < 0.05 and **P < 0.001, compared with control siRNA. [Color figure can be viewed in the online issue, which is available at http://www.interscience.wiley.com.]