Fig. 4.
Meth and/or gp120 modulates the ZO-1 expression in BMVEC as measured by FACS analysis. BMVEC (1×106 cells/ml) were treated in vitro with Meth (10 nM) ± gp120 (50 ng) for 48 h. The number of ZO-1 expressing BMVEC cells was quantitated using FACS analysis. Stained cells were subjected to light scatter analysis and a fixed population of cells was gated after quadrant markers were set, based on the isotype control and represented as FL-1 (FITC-labeled) on the X axis. Cells positive for ZO-1 were expressed as a percentage of the total cells gated. a: Dot plot presentation of data from a representative experiment. b: Graphical representation of data expressed as the mean±SD of 3 separate experiments done in triplicate. Statistical significance was determined using ANOVA based on comparison with the untreated control. Our results show that Meth and gp120 alone and in combination significantly decreased ZO-1 expression.