Table 2.
Competition binding of the hit compounds to mammalian expressed adenosine receptor subtypes A2A and A1.
| Displacement of radioligand at A2AAR-HEK membranes |
Displacement of radioligand A1AR-CHO membranes |
A2A / A1 selectivity |
|||||
|---|---|---|---|---|---|---|---|
| ID |
Ki, µM |
(95% Confidence Interval), µM |
Max Displacementa, % |
Ki, µM |
(95% Confidence Interval), µM |
Max Displacementa, % |
fold |
| 6 | 0.06 | (0.03–0.12) | 48 | 0.09 | (0.04–0.19) | 32 | 1.42 |
| 8 | 1.14 | (0.67–1.94) | 53 | 0.06 | (0.05–0.08) | 100 | 0.05 |
| 9 | 0.49 | (0.20–1.17) | 41 | 1.44 | (0.56–3.70) | 41 | 2.94 |
| 10 | >10 | ND | ND | 1.12 | (0.44–2.88) | 28 | ND |
| 14 | 0.06 | (0.05–0.09) | 54 | 0.14 | (0.04–0.52) | 25 | 2.24 |
| 15 | 0.56 | (0.31–0.99) | 73 | 0.48 | (0.12–1.96) | 27 | 0.87 |
| 17 | 0.72 | (0.55–0.95) | 73 | >10 | ND | ND | >10. |
| 21 | 3.01 | (0.69–13.1) | 57 | 0.39 | (0.31–0.50) | 86 | 0.13 |
| 26 | 0.10 | (0.08–0.11) | 100 | 0.26 | (0.21–0.33) | 100 | 2.74 |
| 29 | 0.11 | (0.09–0.14) | 100 | 0.28 | (0.23–0.33) | 100 | 2.51 |
| 35 | 0.20 | (0.15–0.25) | 100 | 0.15 | (0.13–0.17) | 100 | 0.75 |
| 49 | 0.94 | (0.78–1.13) | 100 | 0.19 | (0.16–0.22) | 100 | 2.01 |
| 50 | 0.15 | (0.11–0.21) | 50 | 1.75 | (0.45–6.81) | 27 | 11.42 |
a
Max displacement refers to the maximal displacement of bound radioligand observed using 10 µM of test compound.