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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biomaterials. 2009 Jun 21;30(27):4618–4628. doi: 10.1016/j.biomaterials.2009.05.030

Figure 1.

Figure 1

Cartoon of our bind-and-lock immobilization strategy for VEGF. VEGF is incubated with a heparin-modified surface and allowed to electrostatically bind through the heparin-binding domain of VEGF (A). For covalent immobilization the heparin modified surface also contains a photoreactive group, p-azidobenzoyl, that can be activated post VEGF binding (B). Thus, this approach uses specific electrostatic (non-covalent) interactions first to orient the growth factor through its heparin binding domain (bind) and then a covalent bond to keep it in place (lock).