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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biomaterials. 2009 Jun 21;30(27):4618–4628. doi: 10.1016/j.biomaterials.2009.05.030

Figure 8.

Figure 8

Western blot analysis of PAE/KDR cells (A) and HUVECs (B) brought into contact with VEGF modified surfaces or exposed to soluble VEGF. (A) Top band shows phosphorylated VEGFR-2 (pVEGFR-2), while bottom band shows total VEGFR-2. Plot quantifies phosphorylated VEGFR-2 band intensities and is normalized to total VEGFR-2 for each condition (n = 5 blots). Surface bound VEGF phosphorylates VEGFR-2 to a greater extent than background in the negative control. Soluble VEGF does not significantly increase phosphorylation signal over surface bound VEGF. (B) Top band shows pVEGFR-2 and bottom band shows total VEGFR-2. Plot quantifies phosphorylated VEGFR-2 band intensities and is normalized to total VEGFR-2 for each condition (n = 3 blots, representative blot shown). Both electrostatically and covalently bound VEGF were able to phosphorylate VEGFR-2 in PAE/KDR cells and HUVECs. The symbol * indicates statistical significance to a level of p < 0.05 using one-way ANOVA with a Tukey post-hoc test, which compares all possible pairs.