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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Int J Cancer. 2009 Jan 1;124(1):36–45. doi: 10.1002/ijc.23894

Figure 7.

Figure 7

Nicotine induces disruption of tight junctions to facilitate EMT and invasion. (A) Nicotine downregulates the membrane localization of the tight junction protein ZO-1 in human A549 lung cancer cells and MCF-7, MDA-MB-468 breast cancer cells. Quiescent cells were treated with 1µM nicotine for the indicated time-points. Immunofluorescence analysis demonstrates the reduction in membrane localization of ZO-1 upon nicotine treatment, indicating that nicotine reduces the presence of membrane-bound ZO-1.