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. 2006 Apr 19;26(16):4256–4265. doi: 10.1523/JNEUROSCI.1253-05.2006

Figure 2.

Figure 2.

Addition of the splice insert at S4 reduces recruitment of neuroligin-1/3/4 but not neuroligin-2. A–F, In a fibroblast-neuron coculture assay, COS cells expressing CFP-tagged neurexin-1β without the S4 insert (A, D) and with the addition of the S4 insert (B, E) induced clustering of both neuroligin-1/3/4 (A, B), and neuroligin-2 (D, E) in contacting dendrites. Induced clusters (arrowheads) were negative for the presynaptic antigen synapsin unlike endogenous synapses (arrows). C, F, Only synapsin-positive neuroligin clusters (arrows) were observed under COS cells expressing CFP-tagged neurexin-1β deleted for the LNS domain. G–I, Quantification of the number (G), area (H), and total integrated intensity (I) of synapsin-negative neuroligin (NLG)-1, -3, -4, and -2 clusters underlying transfected fibroblasts revealed reduced induction of neuroligin-1/3/4 clusters by splice site 4-positive neurexin but no change in induction of neuroligin-2 clusters compared with splice site 4-negative neurexin (∗p < 0.05; n = 40 cells each). Scale bar, 10 μm.