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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Cell. 2004 Dec 29;119(7):1013–1026. doi: 10.1016/j.cell.2004.11.035

Figure 3.

Figure 3

Neurexin Clusters Neuroligins but Not Dystroglycan

(A) Coculture of fibroblasts expressing neurexin-1β-CFP with neurons resulted in only endogenous synaptic clusters (arrows) but no induced clusters of β-dystroglycan at contacts with dendrites.

(B and C) Fibroblasts expressing neurexin-1β-CFP cocultured with hippocampal neurons expressing YFP-neuroligin-1 (B) or YFP-neuroligin-2 (C) induced clusters of YFP-neuroligin lacking immunoreactivity for synapsin in contacting dendrites. The transfected neuron is indicated by *.

(D) Fibroblasts expressing neurexin-1β-CFP cocultured with hippocampal neurons expressing YFP-neuroligin-1 induced overlapping clusters of recombinant YFP-neuroligin-1 and endogenous neuroligin-2 in contacting dendrites. Smaller clusters of gephyrin and PSD-95 were apparent within the neuroligin-positive dendrite regions.

(E) Fibroblasts expressing neurexin-1β-CFP cocultured with hippocampal neurons induced clusters of neuroligin-2, gephyrin, and PSD-95 in contacting dendrites. Within the neurexin contact region as assayed by confocal microscopy, gephyrin clusters were nearly all associated with high concentrations of neuroligin-2, whereas the association of PSD-95 clusters with neuroligin-2-immunopositive regions was more variable.

Scale bars (A)-(C) 10 μm, (D) and (E) 5 μm.