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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Cell. 2004 Dec 29;119(7):1013–1026. doi: 10.1016/j.cell.2004.11.035

Figure 5.

Figure 5

Neuroligin Aggregation Alone Is Synaptogenic

Neurons expressing low levels of YFP-neuroligins were exposed to beads bearing antibodies against YFP to aggregate the neuroligins on dendrites independently of neurexin. Aggregation of YFP-neuroligin-1 resulted in coaggregation of PSD-95 but not gephyrin (A), whereas aggregation of YFP-neuroligin-2 resulted in coaggregation of both PSD-95 and gephyrin (B). Aggregates of the postsynaptic proteins induced by neuroligin aggregation lacked immunoreactivity for the presynaptic protein synapsin. Arrows indicate sites of induced aggregation; other such sites are enlarged below (Nlg1, YFP-neuroligin-1; Geph, gephyrin; PSD, PSD-95; Syn, synapsin; Nlg2, YFP-neuroligin-2). Scale bar 5 μm.