Skip to main content
. 2009 Dec 15;115(6):1137–1144. doi: 10.1182/blood-2009-07-230821

Figure 6.

Figure 6

Characterization of putative lin−CD117−/loCD127−CD90− BM-derived T-cell precursors. Flow cytometric analysis of lin−CD117−/loCD127−CD90− cells from BM. Cells were stained with antibodies against lineage markers CD90, CD117, CD127, CD27, and CD135 (A), and Sca-1 (B), CCR9 (C), CCR7 (D), or with P-selectin Ig to reveal expression of PSGL-1 (E). Cells from CCR9-deficient and CCR7-deficient mice were used as controls in panels C and D, respectively. For staining of PSGL-1, P-selectin-Ig was used in the presence (negative control) and absence of 10 mM EDTA (ethylenediaminetetraacetic acid). Numbers indicate frequencies of cells within gates. Data are representative of 2 independent experiments.