Fig. 4.

Effect of cadmium on the 2F4 immunogold labelling in the cortical tissues: (A–D) untreated samples; (E–G) samples treated with Cd. In control seedlings, 2F4 labelling was intense in the core of cell junctions (Ep CJ) of the epidermis (A) and the tricellular junctions (CTJ) of cortical parenchyma (B). In external tangential wall, the gold particles were localized in the outer half of the wall (Ep ETWo; C). In radial walls (RW), only a few scattered gold granules were detected (D). In the presence of Cd, the labelling decreased significantly in the junction of the epidermis (Ep CJ; E) and sub-epidermal layer (SEL CJ; G) but slightly in CTJ (F). More than ten observations were done per CW domain on two or three independent sections. C, Cuticle. Scale bars: (A–D) = 50 nm; (E–G) = 500 nm.