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A) Image of FF-IEF device. B) Schematic of Microfluidic FF-IEF system. Functionalized pH gradient cathode (pK=9.3) and anode (pK=3.6) polyacrylamide gels are polymerized into the gel regions connected to the separation channel. Posts are included in the gel regions for support. The device is 50 mm by 75 mm with the center triangular separation channel of 46 mm as top width and 56 cm in length. All channels and gel sections have a depth of 160 µm.
