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Western blot result of 14-3-3 sigma from HELA whole cell lysate separation. FF-IEF separation conditions: Voltage: 1800 V. Current: 1.2 mA. [Cell lysate]: 0.5 mg/mL. Flow rate: 0.6 mL/hr. Lane markers correspond to marker (M), sample mixture for negative control (N), positive control (P) or device fraction number (high to low pH).
