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. Author manuscript; available in PMC: 2011 Jan 20.
Published in final edited form as: J Am Chem Soc. 2010 Jan 20;132(2):816–823. doi: 10.1021/ja908850p

Table 2.

MICs of β-lactam antibiotics against E. coli strain producing the OIH-1 β-lactamase fused to various leader sequences

Antibiotic MICs (μg/ml) produced by
TEM + OIH OIH TEM OMP none vector
Penicillin G 64 256 64 256 32 32
Penicillin V 256 512 256 512 128 128
Ampicillin 128 256 64 256 4 4
Amoxicillin 256 512 128 512 8 8
Carbenicillin 1024 2048 512 2048 16 16
Ticarcillin 1024 2048 256 2048 8 8
Piperacillin 8 16 2 16 2 2
Cephalothin 8 8 8 8 8 8
Cefotaxime 0.12 0.06 0.12 0.12 0.06 0.06
Ceftazidime 0.5 0.5 0.5 0.5 0.5 0.5
Aztreonam 0.25 0.12 0.25 0.25 0.25 0.25
Imipenem 0.25 0.25 0.25 0.25 0.25 0.25

TEM, leader of the TEM-1 β-lactamase; OIH, leader of the OIH-1 β-lactamase; OMP, leader of the outer membrane protein OmpA; none, no leader; vector, E. coli DH10B with the vector pHF016. MIC = minimum inhibitory concentration.