Table 1.
The effects of silencing IKKα or IKKβ in the expression of innate immune and inflammatory markers
| Fold changes with siRNA transfection |
|||||
|---|---|---|---|---|---|
| Stimulated |
|||||
| Genes | Bacterial stimulation | Unstimulated control | Mock | IKKα | IKKβ |
| CCL20 | P. gingivalis | 1.5 | 110 | 28 | 15 |
| A. actinomycetemcomitans | 1.9 | 7.7 | 9.6 | 19 | |
| F. nucleatum | 1.5 | 10 | 13 | 15 | |
| CXCL5 | P. gingivalis | 5 | 6.8 | 5.9 | 4.9 |
| A. actinomycetemcomitans | 4.8 | 5.1 | 1.9 | 3.4 | |
| F. nucleatum | 5 | 3.3 | 8.2 | 20.8 | |
| CCL4 | P. gingivalis | 0.3 | 3.4 | 0.1 | 0.2 |
| A. actinomycetemcomitans | 0.1 | 30 | 6.6 | 60 | |
| F. nucleatum | 0.3 | 0.3 | 0.3 | 01 | |
| CXCL14 | P. gingivalis | 4.2 | 12.2 | 2.5 | 2.5 |
| A. actinomycetemcomitans | 2.4 | 7.2 | 5.1 | 13 | |
| F. nucleatum | 4.2 | 1.6 | 1.3 | 1 | |
| IL-6 | P. gingivalis | 0.7 | 3.5 | 0.8 | 0.7 |
| A. actinomycetemcomitans | 3.3 | 38 | 53 | 130 | |
| F. nucleatum | 01 | 0.4 | 0.4 | 0.9 | |
| IL-17 | P. gingivalis | 10.4 | 10 | 2.7 | 1.2 |
| A. actinomycetemcomitans | 1 | 37 | 22 | 50 | |
| F. nucleatum | 4 | 1 | 01 | 1 | |
| IL-1R | P. gingivalis | 7 | 36 | 5 | 01 |
| A. actinomycetemcomitans | 11 | 2 | 0.1 | 3 | |
| F. nucleatum | 01 | 3 | 2 | 01 | |
| IL-10R | P. gingivalis | 11 | 23 | 16 | 11 |
| A. actinomycetemcomitans | 4.7 | 2.7 | 1.1 | 4.1 | |
| F. nucleatum | 1.2 | 1.7 | 1.1 | 0.9 | |
| hBD-22 | P. gingivalis | 1 | 11.3 | 4.5 | 7.3 |
| A. actinomycetemcomitans | 1 | 5.4 | 1.7 | 5.4 | |
| F. nucleatum | 1 | 10 | 9.1 | 7.8 | |
1
No spot density detected.
2
Semi-quantitative analysis of RT-PCR using FluorChem.