Figure 5.

¹⁵N NMR spin relaxation time constants (a) T₁, (b) T₂ and (c){¹H}¹⁵N NOE for arginine kinase at ¹⁵N Larmor frequencies 50.65 (red), 60.78 (green), and 81.04 MHz (blue). Individual values are listed in Supplemental Table 1. Error bars on each measurement represent one standard deviation. The T_1rho spin lock time at 50.65 and 60.78 MHz was shorter than optimal for the slow relaxing amide signals in loop L13 resulting in relatively large errors. A schematic diagram of arginine kinase secondary structure is shown at the top with α-helices shown as cylinders, β-strands as arrows, and loops and extended structure as lines.