Table 4.
Maize NCED and ZEP gene mapping and colocation with QTLs for desiccation and/or ABA content
| Gene Name |
EST | IGR IDa HTGS IDb yrGATE IDc |
Map | Bin | MM coord.d | Proj. coord.e | Flanking markers |
QTL colocation |
|---|---|---|---|---|---|---|---|---|
| NCED1 | [GenBank:ZMU95953] E746 |
Vp14 [GenBank:AC230016.2] |
LHFR_Gnp2004 | 1.08- 1.09 |
504 | 466 | bnlg1643- phi055 |
DW_80_3 FW_80_2 Water_80_1 |
| NCED2 | E746 | AZM4_115740 [GenBank:AC217286.3] |
LHFR_Gnp2004 | 1.05- 1.06 |
328 | 306 | umc 1906- umc 67 |
Water_60_1 %DW_60_2 |
| NCED3 | No | AZM4_115695 [GenBank:AC199036.2] |
LHFR_Gnp2004 | 3.05- 3.06 |
193 | 207 | umc1501- dupssr23 |
No |
| NCED4 | No | AZM4_114127 [GenBank:AC212820.3] |
LHFR_Gnp2004 | 3.00 | -108.7 | -125 | Before umc1746 |
No |
| NCED5 | [GenBank:QCD5h12] | AZM4_50254 [GenBank:AC194862.3] |
LHFR_Gnp2004 | 5.06- 5.07 |
364 | 298 | phi087- umc1537 |
%DW_80_3 ABA_embryo_40_3 DW_60_4 |
| NCED6 | No | AZM4_50252 [GenBank:AC190588.3] |
REFMAP050110 | 4.06 | 177 | 177 | bnlg 1621- umc 66a |
%DW_30_2 |
| ZEP1 | QAG5c10 [GenBank:AI977858] |
AZM5_13314 [GenBank:AC194845.3] yrGATE_Zm2 gZEP1 |
REFMAP050110 | 2.04 | 221 | 221 | bnlg1018- bnlg166 |
ABA_embryo_30_1 ABA_embryo_40_2 ABA_endosperm_40_1 Slope_1 |
| ZEP2 | QAG5c10 | AZM5_13312 [GenBank:AC206194.3] yrGATE_Zm10 gZEP2 |
REFMAP050110 | 10.04- 10.05 |
94.5 | 94.5 | bnlg1526- umc259 |
No |
aThe Institute for Genomic Research.
bHTGS: the High Throughput Genomic Sequences.
cyrGATE: Your Gene Structure Annotation Tool for Eukaryotes.
dMap coordinate computed with MapMaker using 'RI self Haldane' options.
eMap coordionate on REFMAP050110 obtained by homothetic projection with BioMercator [30].
Mapping of candidate genes was performed on the LHRF_Gnp2004 population derived from F2xF252 crossing, except for maize NCED6, ZEP1 and ZEP2 genes which were mapped on the IBM population (REFMAP050110 map) because of no polymorphism between F2 and F252 lines. QTL codes are detailed in Table 3.