Table 5.
QTLs from leaf | |||||||
---|---|---|---|---|---|---|---|
Bin | Locus or marker | QTL position (pcM) on REFMAP050110 | Candidate gene | QTLs from kernel | Lebreton et al [39,44,45]. | Tuberosa et al [39,44,45]. | Pelleschi et al [39,44,45]. |
1.06 | bnl5.59 | 305-344 |
NCED2 Aquaporin |
Water_60_1 | ABAL | RWC, ABAX | |
1.08-1.09 | umc39c | 455-463 | NCED1 | DW_80_3, FW_80_2 Water_80_1 |
ΨL | ABAL | RWC, ABAX |
3.05 | umc10 gsy406 |
212-244 | Vp1 | Slope_3 | ABAX | RWC ΨL |
|
3.06 | Gsy224 | 210-220 | NCED3 | ABAL | |||
4.03-4.04 | Gsy431 | 89 (66-97) | PM37 | Slope_4 | RWC | ||
4.05-4.06 | umc66 | 161-189 |
PIP1;2, PIP1;4 NCED6 |
%DW_30 | ABAL | ABAL | ABAL, ΨL ABAX |
7.01-7.02 | gsy113 umc116 |
163-173 179-189 |
PIP1;5, PIP2;1, PIP2;4, PIP2;6 | Water_60_5 ABA_endosperm_30_2 |
stomatal conductance | ABAL | ABAL ABAX |
7.03 | umc110 | 234-244 253-312 |
PSY3 | ABA_embryo_80_2 ABA_endosperm_80_1 |
stomatal conductance | ABAL | RWC |
9.04 | umc114 | 108-149 | Rab17-EST | %DW_80_5 ABA_embryo_60_1 |
ABAL | ΨL | |
9.07 | gsy330 umc94 |
262-314 | CCD-EST | %DW80_6 ABA_endosperm_80_2 |
ABAX |
For kernel, the LHRF_F3:4 segregating population derived from F2 × F252 crossing. For leaf, populations derived from Polj17 × F2 crossing [39,44,45], high ABA × low ABA lines [39,44,45] and F2 × MBS847 RILs [39,44,45]. Plants were submitted to water deprivation at comparable young stage in [39,44,45] and [39,44,45]. In [39,44,45], ABA concentration was determined in leaf samples from stressed plants irrigated with 50% normal rainfall during stem elongation and before anthesis. Gene codes are detailed in Tables 3 and 4. Genes in bold were mapped by PCR in this study (Table 4 and Additional file 2). Genes involved in kernel drying and located in the vicinity of the QTL confidence interval, are indicated in grey. ABA_embryo: [ABA] in embryo; ABA_endosperm: [ABA] in endosperm; ABAL: leaf [ABA] ABAX: xylem [ABA]; DW: kernel dry matter weight (mg/kernel); FW: kernel fresh matter weight (mg/kernel); %DW = DW/FW× 100; Slope: regression line slope of (Water/FW × 100) as a function of thermal time; RWC: relative water content; Water: kernel water content (mg/kernel); ΨL: leaf water potential.