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. 2010 Feb 23;5(2):e9367. doi: 10.1371/journal.pone.0009367

Figure 2. Analysis of the integrity of mitochondria in DJ-1 deficient and DJ-1 WT MEF.

Figure 2

(A) To determine intramitochondrial ROS production, DJ-1 WT and DJ-1 KO cells were treated with MitoSox® (Invitrogen, USA) according to the manufacturer's instructions for 15 min at 37°C. Fluorescence emission was determined by FACS analysis. An increased intramitochondrial ROS production was observed in DJ-1 KO MEF compared to controls (p<0.05, Student's t-test). Mitochondrial membrane integrity was assessed in DJ-1 WT and KO MEF and in DJ-1 KO cells stably transfected with either DJ-1 WT or the corresponding empty control vector, respectively. The MMP was determined after treatment with TMRM (Invitrogen, USA) for 15 min. at 37°C. The absorption of TMRM in the mitochondria was used as a marker for the integrity of MMP and determined by FACS analysis. (B) A significantly decreased MMP was found in DJ-1 KO cells compared to controls (*p<0.001, Student's t-test). (C) Complementation of the DJ-1 KO phenotype with physiological DJ-1 significantly increased the MMP compared to empty vector control (*p<0.05, Student's t-test).