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. 2009 Jan 6;76(2):91–100. doi: 10.1159/000188664

Fig. 2.

Fig. 2.

Nicotinamide (Nam) affects RA- or D3-induced functional differentiation. Cells treated with Nam plus RA (N/RA) or D3 (N/D3) underwent slower functional differentiation at the early time point (48 h), but showed faster differentiation at the later time point (72 h) compared to treatments with RA or D3 alone by using a functional differentiation marker, DCF. The percentage of positive cells for 48 and 72 h after treatment for control (C), RA, N/RA, D3, N/D3, and Nam (N) is shown. Cells were incubated in PBS containing DCF and TPA and analyzed by flow cytometry. Gates to determine percent positive cells were set to exclude 95% of control cells. a DCF expression levels in cells treated with RA, N/RA, D3 and N/D3 were significantly different from that in untreated cells; b p ≤ 0.05, N/RA or N/D3 treatment was significantly different from treatments with RA or D3 alone.