Skip to main content
. 2009 Jan 6;76(2):91–100. doi: 10.1159/000188664

Fig. 3.

Fig. 3.

Nicotinamide (Nam) accelerated G0 arrest. a Representative histograms of percentage of cells with G1/G0 DNA after 48-hour treatments with RA, Nam plus RA (N/RA), D3, Nam plus D3 (N/D3), and Nam (N), and untreated (control, C). b Nicotinamide enhanced G0 arrest after 48- and 72-hour treatment. The percentage of G1 DNA cells at 48 and 72 h after treatment for control (C), RA, N/RA, D3, N/D3, and N is shown. Cells stained with hypotonic staining solution were incubated for 1 h and analyzed by flow cytometry. a Percentage of G1 in cells treated with RA, N/RA, D3 and N/D3 was significantly different from that in untreated cells; b p ≤ 0.05, N/RA or N/D3 treatment was significantly different from treatments with RA or D3 alone. c HL-60 cells upon nicotinamide treatments grew slowly compared to cells treated with RA or D3 alone or untreated cells.