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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: Exp Cell Res. 2009 Dec 21;316(5):716–727. doi: 10.1016/j.yexcr.2009.12.008

Fig. 3.

Fig. 3

(A) Apoptotic and necrotic labeling by PI (propidium iodide) and Alexa Fluor-488 conjugated annexin V for cells in control, 0.7 mM palmitatate (P), 0.7 mM palmitatate supplemented with 100 μM IBMX (P+I), 0.7 mM palmitatate supplemented with 1 μM forskolin (P+F), 0.7 mM palmitatate supplemented with 1 μM 8CPT-2Me-cAMP (P+8CPT), 0.7 mM palmitatate supplemented with 100 nM glucagon (P+G) and 0.7 mM palmitatate supplemented with 10 μM forskolin and 100 μM IBMX (P+FI) for 24 hrs. Early apoptotic cells: PI− annexin V+ cells; late apoptotic cells: PI+ annexin V+ cells; necrotic cells: PI+ annexin V− cells (n=3). (B) Apoptotic and necrotic labeling by PI (propidium iodide) and Alexa Fluor-488 conjugated annexin V for cells in control, control supplemented with FI (ctrl+FI), 0.4 mM palmitate (0.4P), 0.4 mM palmitate supplemented with FI (0.4P+FI), 0.7 mM palmitate (0.7P), 0.7 mM palmitate supplemented with FI (0.7P+FI), 0.7 mM oleate (0.7O) and 0.7 mM oleate supplemented with FI (0.7O+FI) for 24 hrs (n=3).