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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: Exp Cell Res. 2009 Dec 21;316(5):716–727. doi: 10.1016/j.yexcr.2009.12.008

Fig. 5.

Fig. 5

(A-H) Triglyceride storage by Oil Red O staining for cells in (A) control, (B) 0.7 mM oleate, (C) 0.7 mM linoleate, (D) 0.7 mM palmitate, (E) control supplemented with 10 μM forskolin and 100 μM IBMX (FI), (F) 0.7 mM oleate supplemented with FI, (G) 0.7 mM linoleate supplemented with FI and (H) 0.7 mM palmitate supplemented with FI for 24 hrs (n=3). (I) Quantification of triglyceride levels by an assay for cells in control, 0.7 mM oleate and 0.7 mM palmitate without (w/o) or with (w/) FI for 24 hrs (n=4). (J) Apoptotic and necrotic labeling by PI (propidium iodide) and Alexa Fluor-488 conjugated annexin V for cells in 0.7 mM palmitate and 0.7 mM palmitate supplemented with FI in the absence or presence of free fatty acid oxidation inhibitor etomoxir (n=3). *: p<0.05.