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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: Exp Cell Res. 2009 Dec 21;316(5):716–727. doi: 10.1016/j.yexcr.2009.12.008

Fig. 8.

Fig. 8

(A) Caspase 3 activity of cells in control (ctrl), 0.2 mM palmitate (0.2-P), 0.4 mM palmitate (0.4-P), 0.7 mM pamitate (0.7-P), 0.7 mM oleate (0.7-O) and 0.7 mM linoleate (0.7-L) without (w/o) or with (w/) FI for 24 hrs (n=4). (B) Apoptotic and necrotic labeling by PI (propidium iodide) and Alexa Fluor-488 conjugated annexin V for cells in control (ctrl) and 0.7 mM palmitatate (P) in the absence or presence of FI and/or 10 μM pan-caspase inhibitor Z-VAD-FMK (ZVAD) for 24 hrs. Early apoptotic cells: PI− annexin V+ cells; late apoptotic cells: PI+ annexin V+ cells; necrotic cells: PI+ annexin V− cells (n=3). *: p<0.05; **: p<0.01; ***: p<0.001.