Figure 4.
Variability in density-induced aromatase expression among ASCs from different individuals. ASCs from different disease-free individuals were acquired and subjected to culturing under sub-confluent and confluent conditions. After 10 days of culturing aromatase mRNA (A) and activity assay (B) were measured. Due to undetectable levels of aromatase enzymatic activity in the sub-confluent samples, only the confluent samples were analyzed in the enzyme assay. Same ASCs were treated with adipogenic medium, stained with Oil-Red-O, and percentages of positively stained cells were presented (C).
