Fig. 5.
Characterization of microglia by double immunolabeling with Iba1 and ED1 in spinal cord ventral horn (A-D) and brainstem (E, F) shows increased ED1 immunoreactivity present in some but not all activated microglia. Control tissue shows a lack of ED1 staining in non-activated ramified microglia stained with Iba1 (A, C, E). ED1 staining is prominent in virus-infected spinal cord and brainstem, merged images (B, D, F) and colocalized mostly with activated microglia (D). However, not all activated microglia are ED1-positive. Arrows in D and F indicate activated microglia lacking ED1 staining. Asterisks indicate central canal. Scale bar, 200 μm in B for A and B, 50 μm in D for C and D, 100 μm in F for E and F.