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. Author manuscript; available in PMC: 2011 Feb 9.
Published in final edited form as: Toxicology. 2009 Dec 23;268(3):176–183. doi: 10.1016/j.tox.2009.12.010

Figure 2.

Figure 2

(A) Cas IIgly (6 h treatment) produced a dramatic loss (>70%) in intracellular glutathione (GSH) levels in A549 and H157 cells using 5 μM and 2.5 μM Cas IIgly, respectively, compared to control (Co). The 100% values for both cell lines were around 90 nmoles/mg of protein (±10% from one experiment to another). (B and C) Using membrane integrity (percentage of LDH release) as an index of cytotoxicity (24 h treatment), Cas IIgly was more cytotoxic in H157 cells compared to A549 cells (IC50s 3 μM and 6 μM, respectively). In both cell lines, the cytotoxicity was potentiated by the GSH synthesis inhibitor BSO (20 μM).