Fig. 6.

hVps45 regulates cell migration. (A–E) Human foreskin fibroblast cells were grown on coverslips coated with fibronectin. Cells were either Mock-treated or treated with hVps45-siRNA for 48h. Scratches (wounds) approximately 850 μm wide were introduced onto the coverslips using a pipet tip. Fields of cells were fixed immediately after introducing the scratch (A, C), whereas the cells shown in B and D were fixed 6 h later. Cells were immunostained with 488-conjugated anti-wheat germ agglutinin antibody, which marked the outlines of the cells. (E) Quantification of three independent experiments as performed in A–D. Error bars display standard error and asterisk denotes p-values less than 0.05.