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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jan 4;1801(4):496–502. doi: 10.1016/j.bbalip.2009.12.013

Figure 4. Regulation of PPARα and HNF4α transcriptional activity by PC-TP in vitro.

Figure 4

(A) Influence of PC-TP expression on PPARα transcriptional activity. HEK 293T cells were treated with scrambled siRNA (open bars) or PC-TP siRNA (closed bars). PPARα transcriptional activity was determined according to firefly luciferase activity expressed by PPRE3-tk-luc in the absence (−) or presence (+) of exogenous PPARα expression (pcDNA-hPPARα), as well as BSA or DHA complexed to BSA (DHA/BSA). The inset western blot shows the effects of PC-TP siRNA treatment compared with scrambled siRNA or non-transfected control cells. (B) Influence of PC-TP expression on HNF4α transcriptional activity. HEK 293T cells were treated with siRNA as described in panel A. HNF4α transcriptional activity was determined according to firefly luciferase activity expressed by HNF4α-TK-luc in the absence (−) or presence (+) of exogenous HNF4α expression (pCMX-HNF4α). Data are representative of three independent experiments, each experiment was performed in triplicate. *P < 0.05, PC-TP vs. scrambled siRNA.