Table 1.

Genes Involved in Lipid and Glucose Metabolism that are Differentially Expressed in Fenofibrate Fed Pctp^−/− vs. Wild Type Mice^a

Gene	Abbreviationb	Affymetrix ID	dChipc	BADGEc	qPCRd
Triglyceride Metabolism
Fibroblast growth factor 21e	FGF21	1422916_at	3.00	2.95	11.22
Lipase, endotheliale		1421262_at,    1450188_s_at	2.09	2.17
Phosphatidic acid    phosphatase 2A		1422620_s_at	1.56	1.49
Stearoyl-Coenzyme A    desaturase 2		1415823_at	1.55
Aquaporin 9		1421605_a_at	1.47	1.62
ATP-binding cassette, sub-    family D (ALD), member 2		1419748_at	1.45	1.72
Glycerol-3-phosphate    acyltransferase,    mitochondrial		1425834_a_at	1.39	1.54
Acyl-CoA synthetase long-    chain family member 1e		1422526_at		1.22	5.80
Fatty acid synthase	FAS	1423828_at	1.07f		4.30
Apolipoprotein M		1419095_a_at,    1419096_at	−1.41	−1.40
Lipin 2		1452837_at	−1.47
Carnitine palmitoyltransferase    1a, liver	CPT1a	1434866_x_at	−1.53
Lipin 1		1418288_at,    1426516_a_at	−2.40	−2.08
Acetyl-coenzyme A    carboxylase alpha	ACCα	not present			2.39
Cholesterol and Phospholipid Metabolism
NAD(P) dependent steroid    dehydrogenase-like		1451799_at	2.78
Acetoacetyl-CoA synthetase		1423797_at,    1456081_a_at	2.63	3.08
3-hydroxy-3-methylglutaryl-    Coenzyme A reductasee		1427229_at	1.78	2.07	2.34
Low density lipoprotein    receptore	LDLr	1450383_at,    1421821_at	1.75	1.96
ATP-binding cassette, sub-    family A (ABC1), member    1e	ABCA1	1421839_at,    1450392_at	1.75	2.03
Phosphatidylserine synthase    1	PSS1	1441866_s_at		1.53
Mevalonate (diphospho)    decarboxylase		1417303_at,    1448663_s_at	1.68
Squalene epoxidasee		1415993_at	1.39		2.33
Sterol-C4-methyl oxidase-like		1423078_a_at	1.38
Phosphate    cytidylyltransferase 1,    choline, alpha isoform	CTα	1424453_at	1.34	1.44
Phosphatidylinositol transfer    protein, cytoplasmic 1		1452940_x_at,    1455204_at	−1.51
ATP-binding cassette, sub-    family G (WHITE),    member 8	ABCG8	1420656_at	−1.69	−1.55
Glucose Metabolism
Glucokinasee	GK	1419146_a_at,    1425303_at	2.76	2.43
Ribose 5-phosphate    isomerase A		1418337_at	1.57	1.49
Dihydrolipoamide S-    acetyltransferase (E2    component of pyruvate    dehydrogenase complex)		1452005_at		1.43
Isocitrate dehydrogenase 3    (NAD+) alpha		1432016_a_at		1.40
Phosphoglucomutase 1		1453283_at		1.37
Pyruvate dehydrogenase E1    alpha 1		1449137_at	1.34
6-phosphofructo-2-    kinase/fructose-2,6-    biphosphatase 1		1427213_at	−1.41
Solute carrier family 2    (facilitated glucose    transporter), member 1		1434773_a_at	−1.55
Insulin receptor substrate 2	IRS2	1443969_at	−1.81
Phosphoenolpyruvate    carboxykinase 1,    cytosolice	PEPCK	1423439_at	−1.88	−2.63
Glutamate oxaloacetate    transaminase 1, soluble		1450970_at	−2.67
Transcriptional Factors
Nuclear receptor subfamily 0,    group B, member 2e	SHP	1449854_at	2.26
Sterol regulatory element    binding factor 1	SREBP1c	1426690_a_at	1.62	1.67	14.06
Nuclear receptor subfamily 3,    group C, member    1(glucocorticoid receptor)		1421866_at	1.59	1.49
Retinoic acid receptor, beta		1454906_at	1.53	1.44
Hypoxia inducible factor 1,    alpha subunit	HIF-1α	1448183_a_at	1.50	1.74
Hepatic nuclear factor 4,    alphae	HNF4α	1421983_s_at	1.49	1.60	6.84
Sterol regulatory element    binding factor 2e	SREBP2	1426744_at	1.47		9.60
Nuclear receptor subfamily 2,    group F, member 2    (COUP-TFII)		1416158_at	1.34	1.78
Peroxisome proliferator    activated receptor alphae	PPARα	1449051_at	−1.36
Nuclear receptor subfamily 5,    group A, member 2e	LRH-1	1449706_s_at	−1.41
CCAAT/enhancer binding    protein (C/EBP), delta		1423233_at	−1.56
Forkhead box O1e	FoxO1	1416982_at,    1416983_s_at	−1.60	−1.51
Forkhead box O3a		1434831_a_at,    1434832_at	−1.68	−1.61
Peroxisome proliferative    activated receptor,    gamma, coactivator 1 beta	PCG-1β	1449945_at	−2.35	−1.85
Peroxisome proliferative    activated receptor,    gamma, coactivator 1    alphae	PCG-1α	1437751_at,    1456395_at,    1460336_at	−3.15	−2.21

^aData are expressed as ratios of hepatic gene expression values for Pctp^−/− mice/wild type mice after feeding a fenofibrate-supplemented chow diet for 7 d as determined by dChip or BADGE algorithms. Selected genes were tested by quantitative real-time PCR (qPCR) for differential expression. Genes were assigned to categories using GenMAPP.

^bCommon gene abbreviation used in the text.

^cWhere more than one Affymetrix ID is listed, values represent averages of fold changes.

^dqPCR was performed (wild type, n = 6 – 7; Pctp^−/−, n = 4 – 6) and differences were determined to be significant at P < 0.05.

^eqPCR was performed using cDNA prepared from livers of chow fed mice (wild type, n = 4; Pctp^−/−, n = 4), which revealed no significant difference for these genes.

^fFalls below the threshold for significance by dChip algorithm.