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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Orthop Res. 2010 Apr;28(4):529–536. doi: 10.1002/jor.21014

Fig. 4. Non-muscle NIH3T3 cells do not promote cartilage matrix production in 2D cultures.

Fig. 4

A. Collagen II immunostaining (red) after co-culturing RCS chondrocytes with NIH3T3 cells for 4 days. Scale bars, 20μm. B. Quantification of Col II fluorescent intensity using the program Image J. Values are normalized to total number of chondrocytes.. * denotes: P<0.05 in statistical analysis (n=4). C. Quantification of alcian blue staining from 3 culture replicates. Values shown here are the absolute values of absorbance reading at 590nm when dissolved in 4M GuCl.. * denotes: P<0.05 in statistical analysis.