Table 1.
Cell type | Supplementation or treatment | Concentrations used | Time of treatment | Experiment or assay |
---|---|---|---|---|
HEK-293T | CdCl2 ZnCl2 |
10; 20μM 50; 100μM |
20 h 20 h |
Luciferase |
HEK 293T MSTO-211H |
CdCl2 ZnCl2 |
20μM 100μM |
1h* | ChIP, mRNA |
WISH | CdCl2 ZnCl2 |
20μM 100μM |
1h* | mRNA |
MTF-1 FLAG MTF-1-KO |
CdCl2 ZnCl2 |
10μM 50μM |
30 min* | mRNA |
After the indicated time of treatment media was exchanged to media without metals and incubated for an additional 2 hours prior to RNA isolation. For ChIP assays this step was omitted.