. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: Biochim Biophys Acta. 2009 Nov 12;1800(3):416–424. doi: 10.1016/j.bbagen.2009.11.003

Table 1.

Treatment conditions in RT-PCR, ChIP and luciferase assays.

Cell type	Supplementation or treatment	Concentrations used	Time of treatment	Experiment or assay
HEK-293T	CdCl₂ ZnCl₂	10; 20μM 50; 100μM	20 h 20 h	Luciferase
HEK 293T MSTO-211H	CdCl₂ ZnCl₂	20μM 100μM	1h^*	ChIP, mRNA
WISH	CdCl₂ ZnCl₂	20μM 100μM	1h^*	mRNA
MTF-1 FLAG MTF-1-KO	CdCl₂ ZnCl₂	10μM 50μM	30 min^*	mRNA

After the indicated time of treatment media was exchanged to media without metals and incubated for an additional 2 hours prior to RNA isolation. For ChIP assays this step was omitted.