Fig. 5.

The apoptosis and relative expression level of Bcl-2 mRNA in porcine IVF and SCNT embryos during development in vitro. A1 and A2 shown the apoptotic rate of IVF and SCNT embryos, respectively; B1 and B2 shown the relative Bcl-2 mRNA levels of IVF and SCNT embryos, respectively. The experiment was replicated three times. dates are presented as mean ± SEM. a,b Values with different letter superscripts are significant difference(P < 0.05) in the relative Bcl-2 transcript abundance between in IVF and SCNT embryos at given stage of development; Bars with an asterisk(*) are significantly difference(P < 0.05) in apoptotic rate between in IVF and SCNT embryos at given stage of development; ‘n’ is the number of embryos tested by comet assay