Table 1.
Chromosome analysis of IVF zygotes and ICSI zygotes derived from MβCD-treated spermatozoa
| Fertilization method | Sperm treatment with MβCD | No. of zygotes analyzed (no. exp.) | No. of aneuploid zygotes (%) | No. of zygotes with structural chromosome aberrations (%) | No. of aberrant chromosomes | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome type | Chromatid type | ||||||||||||
| Break | Gap | Dicentric | Translocation | Ring | Break | Gap | Exchange | Total | |||||
| IVF | Control | 253 (4) | 2 (0.8) | 4 (1.6) | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 4 |
| IVF | 1 mM, 2 h | 211 (5) | 3 (1.4) | 6 (2.8) | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 |
| ICSI | 0 mM, 3–3.5 h | 212 (6) | 6 (2.8) | 23 (10.8)a | 15 | 4 | 16 | 0 | 1 | 10 | 0 | 0 | 46 |
| ICSI | 1 mM, 3–3.5 h | 200 (7) | 5 (2.5) | 9 (4.5)b | 2 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 9 |
aSignificantly high (P < 0.001) compared to control IVF zygotes derived from spermatozoa incubated without MβCD
bSignificantly low (P < 0.05) compared to ICSI zygotes derived from spermatozoa incubated without MβCD