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. 2009 Dec 10;61(4):995–1002. doi: 10.1093/jxb/erp363

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — BABA provokes a stress-induced morphogenic response in Arabidopsis. (A) BABA affects cell cycle activity of the root meristem. Cell cycle activity was evaluated by measuring primary root tip proCYCB1;1:GUS reporter activity (Negi et al., 2008). Five-d-old seedlings were vertically grown in the presence of water or 500 μM BABA and stained for 2 h for GUS activity. (B) Effects of BABA on lateral root density. Lateral root density was evaluated on 15-d-old in vitro plants vertically grown on half-strength MS medium supplemented with 75 μM BABA or water (control). The data are means ±SE (n >30). (C) Comparison of primary root length of 15-d-old Arabidopsis seedlings vertically grown on half-strength MS medium containing 75 μM BABA with the water control. (D, E) BABA inhibits Arabidopsis growth. Fresh weight (D) and size (E) were evaluated 3 d after water (control) or 300 μM BABA treatments. Error bars are SD. All experiments were repeated at least three times. Representative results are shown. (B, C, D) The asterisk indicates significant difference (P <0.01, Student's t-Test).