Fig. 2.

BABA induces accumulation of anthocyanin. Foliar anthocyanin accumulation was observed in 4-week-old (A) or in 5-week-old (B) Arabidopsis. The asterisk indicates significant difference (P <0.01, Student's t-Test). (C) Effect of BABA on the transcript levels of CHS and DFR genes in Arabidopsis. qRT-PCR relative expression levels were monitored in 3-week-old Arabidopsis. EF-1-ALPHA was used as an internal standard control. Expression levels of BABA-treated Arabidopsis were compared to water-treated controls (defined value of 1). Error bars are SD (n=3 technical replicates). For all experiments, Arabidopsis were treated with water (control) or 200 μM BABA and samples were collected 48 h later. Experiments were repeated twice with similar results.