Fig. 3.

Expression profile of fragments encoding expansins sequences of wheat grain pericarps. Four expression patterns were identified: A. Variable expression (no single peak was identified); B. Expression decrease gradually; C. Peak to 61°Cd after anthesis; D. Peak to 186 °Cd after anthesis. Total RNA (2 ng) from grain position 2 collected between 24 °Cd and 325 °Cd after anthesis (2–20 DAA) in growing season 1 was used as a template for RT-PCR analysis by gene-specific primers. The PCR products were separated on a 1% agarose gel and stained with ethidium bromide.