Table 1.
Accession number of cloned sequence | Cloned sequence | Accession number | Expansin gene | Identity with expansin gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
FN556064 | pTaExpA1 | AY589583 | TaExpA1 | 77% | GTG CGG TCA GTG CTA CAA GA | ATA GTT AGG CGG GCA AAG G |
FN556065 | TaExpA2 | AY589584 | TaExpA2 | 98% | CCA CCA TGA TGT GTT GTT CC | AGT AGG AGT GGC CGT TGA TG |
FN556066 | pTaExpA4 | AY543530 | TaExpA4 | 87% | AAC TTC TGC CCG TCG AAC TA | CCC TTC ATG GTG AAC CTC AT |
FN556067 | pTaExpA5 | AY543531 | TaExpA5 | 74% | ACC ACA TCC ACA CAC GAG AG | CCA CCA GCT CGA AGT AGT CC |
FN556069 | pTaExpA6 | AY543532 | TaExpA6 | 92% | CAA TCC TCC CCG CGA AC | GGT CCC CTT CAC CGA CAT |
FN556070 | pTaExpA6 (a)a | AY543532 | TaExpA6 | 84% | GTG CAA CCC TCC TCG ACA C | GGT CCC CTT CAC CGA CAT |
FN556071 | pTaExpA6 (b)a | AY543532 | TaExpA6 | 84% | GCA ACC CTC CCC GCG TC | GGT CCC CTT CAC CGA CAT |
FN556068 | pTaExpA8 | AY543534 | TaExpA8 | 94% | ACT ACG CAC TCC CCA ACA AC | AGA GCT CAA GTC ACC GAT GC |
18S rRNA | GTG ACG GGT GAC GGA GAA TT | GAC ACT AAT GCG CCC GGT AT |
The table shows the maximum identity between sequences cloned and wheat expansins reported.
(a) and (b) represent groups of sequences with homology to TaExpA6 but different between them. (a) has 88% identity with (b).