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. 2010 Jan 15;61(4):1147–1157. doi: 10.1093/jxb/erp380

Table 1.

Primers used for the amplification of cDNA fragments encoding putative expansins of wheat pericarps cv. Bacanora

Accession number of cloned sequence Cloned sequence Accession number Expansin gene Identity with expansin gene Forward primer sequence (5′–3′) Reverse primer sequence (5′–3′)
FN556064 pTaExpA1 AY589583 TaExpA1 77% GTG CGG TCA GTG CTA CAA GA ATA GTT AGG CGG GCA AAG G
FN556065 TaExpA2 AY589584 TaExpA2 98% CCA CCA TGA TGT GTT GTT CC AGT AGG AGT GGC CGT TGA TG
FN556066 pTaExpA4 AY543530 TaExpA4 87% AAC TTC TGC CCG TCG AAC TA CCC TTC ATG GTG AAC CTC AT
FN556067 pTaExpA5 AY543531 TaExpA5 74% ACC ACA TCC ACA CAC GAG AG CCA CCA GCT CGA AGT AGT CC
FN556069 pTaExpA6 AY543532 TaExpA6 92% CAA TCC TCC CCG CGA AC GGT CCC CTT CAC CGA CAT
FN556070 pTaExpA6 (a)a AY543532 TaExpA6 84% GTG CAA CCC TCC TCG ACA C GGT CCC CTT CAC CGA CAT
FN556071 pTaExpA6 (b)a AY543532 TaExpA6 84% GCA ACC CTC CCC GCG TC GGT CCC CTT CAC CGA CAT
FN556068 pTaExpA8 AY543534 TaExpA8 94% ACT ACG CAC TCC CCA ACA AC AGA GCT CAA GTC ACC GAT GC
18S rRNA GTG ACG GGT GAC GGA GAA TT GAC ACT AAT GCG CCC GGT AT

The table shows the maximum identity between sequences cloned and wheat expansins reported.

a

(a) and (b) represent groups of sequences with homology to TaExpA6 but different between them. (a) has 88% identity with (b).