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. 2010 Jan 29;61(4):1215–1224. doi: 10.1093/jxb/erp396

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Temporal and spatial patterns of TRO gene expression. (a–d) RNA in situ hybridization confirmed the expression of TRO during early embryogenesis. (a) Nomarski micrographs showing a strong signal in the embryo proper (EP) and suspensor (S) at the eight-cell stage. (b) Hybridization with a sense probe at the eight-cell stage. (c) Nomarski micrographs showing a strong signal at the heart stage. (d) Hybridization with a sense probe at the heart stage. (e–g) Histochemical assays of the expression pattern of GUS under the control of the TRO promoter in P_TRO–GUS transgenic Arabidopsis. (e) GUS staining of a 7-d-old seedling showing TRO expression in the cotyledons, shoot base and root. (f) GUS staining of a 14-d-old seedling showing TRO expression in cotyledons and hydathodes in a rosette leaf. (g) GUS staining in adult flowers showing TRO expression in pollen grains (inset), anthers, and sepals. Scale bar: a–d, 20 μm; e–f, 1 mm; g, 0.5 mm.