Representative gel of bile samples after polymerase chain reaction (PCR) amplification using Helicobacter-specific primers C97F and C98R. Four water samples were used as a negative control; HP26, a known Helicobacter pylori sample, was used in duplicate as a positive control. In this gel, A, B, F and G were all considered positive and I was considered indeterminate. G and I were considered negative upon repeated PCR experiments. All other samples were considered negative for Helicobacter spp.