Figure 2.

GF2 enhanced maturation of lipopolysaccharide (LPS) -stimulated dendritic cells (DCs). On day 6 of the culture, bone marrow-derived non-adherent cells were collected and treated with RPMI-1640, GF2 (100 μg/ml), LPS (1 μg/ml), or combined GF2 and LPS for 48 hr. After incubation, cells were collected and the expressions of CD80, CD86 and major histocompatibility complex (MHC) class II by DCs were analysed by flow cytometry. LPS was used as the positive control. DCs were gated on CD11c⁺ large cells. (a) Values shown in the flow cytometric profiles are the mean fluorescence intensity (MFI) by CellQuestPro software. Values shown were from one representative experiment of four independent experiments performed. (b) The MFI was calculated, and results are expressed as the mean ± SEM from four experiments. *P<0·05 compared with medium-treated DCs.