Skip to main content
. 2010 Mar;129(3):386–395. doi: 10.1111/j.1365-2567.2009.03190.x

Figure 2.

Figure 2

(a) FLQ-specific cytotoxic T lymphocyte (CTL) cultures obtained from three different donors. CTL cultures were obtained after three consecutive stimulations and were then tested in triplicate against untreated or FLQ-pulsed autologous phytohaemagglutinin (PHA) -blasts. Results are expressed as the per cent specific lysis obtained at the indicated effector : target ratio. One representative experiment out of three is shown. (b) FLQ-specific CTL clones, obtained from a polyclonal CTL culture from donor 19, were obtained by limiting dilution. Clones were tested against untreated or FLQ-pulsed autologous PHA-blasts. The cytotoxic activity of the indicated clones is expressed as the per cent specific lysis obtained at the indicated effector : target ratio. One representative experiment out of three is shown. (c) Peptide titration. FLQ-specific CTL clones were tested against autologous PHA-blasts after preincubation with the indicated concentration of synthetic peptides from the Epstein–Barr virus nuclear antigen 1 (EBNA1) region 565–574. The minimal epitope was defined as the 9-mer LQTHIFAEV (EBNA1 566–574). Results are expressed as the per cent specific lysis obtained at an effector : target ratio of 3 : 1. Means ± SD of three independent experiments performed in triplicate are reported.