Figure 6.

Presentation of the LQT epitope by Trojan antigen (TA) delivery. The LQT epitope was linked to the HIV Tat protein transduction domain (RKKRRQRRR) via a furin-sensitive (SL) or furin-resistant (RL) linker, and delivered to autologous lymphocyte cell lines (LCL), BJAB cells or mismatched LCL. Cells were incubated for the indicated time period (overnight or 90 min), and then used as a target of LQT-specific cytotoxic T-lymphocyte (CTL) clones in a standard chromium release assay. LQT-pulsed target cells were also used as control. Results are expressed as per cent specific lysis obtained at an effector : target ratio of 3 : 1. One representative experiment out of three is shown.