Changes in gene expression of (a) CCL-2, CCL-4, CCL-20 and CXCL2 and (b) interleukin-6 (IL-6), IL-8 and tumour necrosis factor-α (TNF-α) in monocyte-derived dendritic cells (MoDCs) and IL-12 in blood dendritic cells (BDCs) following a 6-hr stimulation with lipopolysaccharide (LPS). MoDCs at day 6 and BDCs at day 1 were cultured with LPS (100 ng/ml). Samples were assessed for changes in gene expression by quantitative real-time polymerase chain reaction using ribosomal protein L19 as the reference gene. Results are shown as the median fold change relative to the control (n = 4 animals). a,b,c,dP < 0·05 MoDC versus BDC.