Figure 1.

Induction of killer-cell immunoglobulin-like receptors (KIR) expression on cord blood CD8⁺ T cells in vitro. Frequency of cord blood and adult CD8⁺ T cells expressing CD158b after incubation for 4 days of cord blood mononuclear cells (healthy newborns) and peripheral blood mononuclear cells (healthy adults) in medium alone, medium supplemented with interleukin-2 (IL-2; 1 or 10 ng/ml) and in the presence of plate-bound anti-CD3 monoclonal antibody (αCD3). (a) without additional treatment. (b) without (white histograms) and with (black histograms) treatment with 5-aza-2′-deoxycytidine (5-aza) for 4 days. Mean ± SEM are shown. Data are representative of (a) 7–13 experiments and (b) 3–13 experiments. Statistical analysis was performed using Wilcoxon matched paired test. P-values are indicated.